1% Hyaluronic Acid (Sodium Hyaluronate) Solution Recipe

1% Hyaluronic Acid (Sodium Hyaluronate) Solution Recipe with Preservatives (makes 12.5 fluid ounces or 370 milliliters)

Recommended Serving Amount: 1 Tbsp (about 15 ml) Total Grams: 370.0

 Ingredients and Serving Amounts Recipe Item mg per
Grams Percent 1 Tbs
1 -Hyaluronic Acid (Sodium Hyaluronate) Powder 3.76 1.0% 150
2 -Ascorbic Acid Powder 3.76 1.0% 150
3 -DMAE (Dimethylaminoethanol) Powder 3.76 1.0% 150
4 -EDTA Calcium Disodium Powder 3.76 1.0% 150
5 -12 fluid ounces (355 grams) distilled or filtered water 355 96%

Additional things that you’ll need.

  1. 16 fluid ounce capacity plastic water bottle with tightly sealing lid
  2. plastic or stainless steel funnel fitting water bottle
  3. stainless steel tongs
  4. clean fork
  5. 16 fluid ounce measuring cup
  6. large pot with lid
  7. small sauce pan with lid
  8. exam gloves (nitrile or latex)
  9. facemask
  10. roll of clean paper towels
  11. small clean sheet of printer or wax paper (untouched except at corners)
  12. gram weight scale with accuracy 0.01 gram or better

Introduction:

Hyaluronic Acid is a popular and effective dietary supplement that is used for and may help support joint health and may also help reduce the discomforts associated with some types of joint pain associated with aging. Preparing a 1% solution of Hyaluronic Acid can be both easy and challenging. There are a lot of things that can and probably will go wrong the first few times to attempt such an undertaking. But with diligence and attention to proper sanitary techniques and protocols the results you can achieve with this Do-It-Yourself (DIY) recipe can be especially rewarding.

Why should I prepare a 1% Hyaluronic Acid solution and not 2% or higher?

Hyaluronic Acid (HA) is an interesting dietary supplement. When dissolved in water according to the instructions given it will thicken and absorb up to 500 times its own weight. At a solution concentration of 1% HA will be somewhat viscous and slow pouring. At concentrations above about 1.5% a properly prepared HA solution can be so thick (viscous) that it can become completely gelatinous, impossible to pour and making storage and dispensing difficult or impossible.

Why should I consider adding preservatives to my Hyaluronic Acid solution?

Preparing a Hyaluronic Acid (HA) solution properly is a lot like the home canning of jams or jellies. Perhaps you might recall watching your mother or grandmother prepare preserves and you might also recall a lot of her tedious attention to cleanliness and her occasional exclamations of “Don’t touch that!” That’s because the making of preserves requires following strict sanitation protocols carefully. Just the smallest mistake often resulted in losses due to spoilage caused by inadvertent contamination. Even assuming that during your HA solution preparation you followed the required sanitary protocols perfectly you might still have problems owing to the fact that the dietary supplement ingredients, even when handled properly, may include a few microbes increasing the potential for microbial contamination, colonization and eventual spoilage. Another consideration is that the preservatives that you would be adding with the above recipe are actually dietary supplements with potential health benefits in addition to their acting as microbial growth inhibitors (preservatives).

What will be the expected shelf-life of my Hyaluronic Acid solution?

Making your Hyaluronic Acid (HA) solution with preservatives may extend shelf-life but, unfortunately, it isn’t possible to say with confidence what the shelf-life will be with or without preservatives. Depending upon how careful or lucky you are in keeping your HA solution free from microbial contaminants your unopened and refrigerated solution may last a few weeks or possibly many months. I’ve seen Hyaluronic Acid solutions made with no preservatives last for several weeks and I’ve seen solutions made with preservatives last only a few weeks. One thing that is certain is that if your solution becomes contaminated with a significant number of colonizing microbes or yeast, and it isn’t refrigerated, it’ll likely not last much longer than 24 to 48 hours.

How can I tell if or when my Hyaluronic Acid spoils or goes bad?

Typically when a Hyaluronic Acid solution spoils it will become more watery (less viscous). When going bad the solution can also grow mold on top, become cloudy or develop an unusual smell.

Do you have a recipe for preparing a Hyaluronic Solution without preservatives?

Sure! Just omit the three dietary supplement preservatives from this recipe. Do add the Hyaluronic Acid powder but do not add the Ascorbic Acid, DMAE and EDTA powders.

Can I use fewer than the three preservatives listed such as using only Ascorbic Acid?

Absolutely! Just omit the other two preservatives. However, you may want to consider that some researchers have raised concerns that using Ascorbic Acid as a preservative for HA solutions may lead to degradation (a breaking up and shortening of the long chain HA molecule) when Ascorbic Acid concentrations exceed more than about 2% by weight of the total solution. With this recipe that 2% limit would translate to adding no more than 7.52 grams of Ascorbic Acid.

Can I safely ship a Hyaluronic Solution with preservatives?

Maybe yes, and maybe no. Properly prepared with the above preservatives a Hyaluronic Acid solution should last one week at room temperature or possibly longer. But a lot depends upon your sanitary techniques and the microbial numbers and types in the resulting solution as well as the conditions and temperatures during transport. If shipped during the summer months by ground carrier services you might be pushing the limits of even the best prepared HA solution that includes preservatives.

Directions:

Measure out about 16 fluid ounces of distilled or filtered water and bring to boil in small sauce pan with lid and then turn heat down to low simmer for minimum of 60 minutes (do not remove lid until instructed to do so). After starting the small pot of distilled water simmering fill the large pot about half way with tap water. Fill and submerge the water bottle and lid into the pot but be sure to keep the bottle and lid separated (not sealed). It’s okay if the bottle and or lid are floating slightly. Also add and submerge in the large pot the stainless steel tongs and funnel. Bring the large pot to a boil and then reduce heat to a low simmer with lid for 60 minutes.

You will need to observe sanitary precautions throughout this recipe thereby reducing the likelihood of microbial contamination. So before starting the following steps put on your exam gloves and facemask. After the hour simmering has passed turn off the heat to the small and large pots. Take the pots to your counter space where you can work and place on trivets or heat pads. Place one to two feet of clean paper towel on the counter next to the pots. Remove the lid from the large pot and with a clean fork lift the stainless steel tongs free from the hot water and allow to air cool for about 30 seconds. When cooled sufficiently grab the tongs with your gloved hands and use the tongs to pick up and drain the 16 fluid ounce water bottle and then set upright on the paper towels on the counter. Using the tongs pick up bottle lid and place that on the paper towels.

Using the tongs pick up and drain the 16 fluid ounce measuring cup and place that on the paper towels as well. Remove the lid to the small pot and pour out a liquid measure of 12 fluid ounces of the distilled water into the measuring cup. Using the stainless steel tongs remove the funnel from the large pot and insert into water bottle (you may hold the outside of the water bottle with your gloved hand during this procedure). Using the tongs grab and secure the outside of the bottle (don’t use your hand in case you spill the hot water) and then pour the hot distilled water from the measuring cup into the bottle.

Using your gloved hand remove the funnel from the bottle and secure and tighten the lid to the water bottle. The water bottle should now be sealed and approximately 3/4 full of hot distilled water. Place the funnel and stainless steel tongs back into the large pot, place back onto stove burner and return lid but don’t turn on the burner just yet. Place the sealed water bottle in a brand new gallon sized zip locking plastic bag into the refrigerator and allow to cool overnight.

Next morning you will need to turn on the large pot that includes the funnel and stainless steel prongs and bring to a boil again. You shouldn’t have to boil it for more than a few minutes this time because it should already be mostly sterile from the previous days efforts assuming you kept the lid on overnight and didn’t mess with it too much. Set out another 1 to 2 feet of clean paper towels on your working counter. Put on your exam gloves and facemask again. Remove the lid from the large pot and using a clean fork remove the stainless steel tongs and allow to air dry until cool enough for you to grab with your gloved hand as before. Using the stainless steel tongs remove the funnel from the large pot and place on the paper towels.

Set the tongs down on the paper towels as well and allow the funnel to cool and air dry completely for 5 to 10 minutes. After these are completely dry remove the bagged bottle of distilled water from the refrigerator, unbag and stand upright on the paper towels. Using your gloved hand remove the lid to the bottle of distilled water and place the lid upside down on the towels being careful not to contaminate the interior surfaces of the lid. Using your gloved hand insert the funnel into the water bottle.

When weighing out your ingredients you will need to use a very clean (untouched) spoon and 3 or 4 inch squares of clean (untouched) printer or wax paper placed on your digital scale. Just make sure that everything coming into contact with the powders is completely sanitary or sterile. After placing a new and clean piece of printer or wax paper on the scale tare the scale to read 0.00 grams. Measure out each ingredient separately to the weights indicated above adding them separately to the water bottle by pouring them into and through the funnel. After all ingredients have been added to your water bottle use your gloved hand to tighten and seal the lid on the water bottle. Shake the water bottle for about 5 minutes.

Because the water is very cold the ingredients will only dissolve slowly. But the Hyaluronic Acid Powder in particular will appear rather clumpy and undissolved after your first shaking but do not worry as this is completely expected. Place the water bottle back into the zip lock bag and place back into the refrigerator. Periodically every few hours remove the bagged water bottle from the refrigerator and shake for 1 to 2 minutes. It usually takes up to 24 hours and 4 or more periodic shakings for you to see that the HA has dissolved completely. As the HA gradually dissolves you’ll notice that the solution becomes thicker and more viscous. After 24 hours your solution should be completely clear and viscous, about the same consistency or viscosity of maple syrup, or thicker, but not quite as viscous as honey. It is recommended to keep your Hyaluronic Acid solution refrigerated at all times and be careful when pouring out your servings to avoid touching or contaminating the inside of the lid or the lip of the bottle. Even with refrigeration and the above added preservatives your HA solution can and probably will spoil eventually.

PureBulk offers all of the bulk powder dietary supplement ingredients for this recipe on our website at competitive prices. Enjoy!

Comments

comments